Developing ctDNA analytics to improve precision healthcare

We offer comprehensive genomic testing for healthcare, academia and pharmaceutical companies. Our ctDNA analysis pipeline is developed in an academic context and has been used to analyze over 2,000 blood samples. With Business Finland TUTLI funding, we are now translating our technology into a commercial offering to benefit cancer patients.

Process

Sample collection

Two 10ml blood samples are collected into Streck cell-free DNA BCT tubes at regional laboratories and shipped to our facilities in Finland. Sample shipping is free of charge.

DNA analysis

We extract cell-free DNA from the samples and sequence it using a panel capturing cancer associated genes. We computationally analyze millions of DNA fragments found in the patient's blood to construct a map of the genomic alterations driving the cancer.

Reporting

We deliver a detailed report including information about genomic alterations, a list of identified biomarkers, available therapies, and relevant clinical trials. The report is delivered within 14 days of receiving the sample.

Cancer specific panels

Our tailored panels encompass all genes of clinical interest in a specific cancer type. Cheaper and more informative than a pan-cancer panel, they are an optimal choice for patients with a known diagnosis. Starting price is 1200€ per sample, but discounts apply for larger orders. We currently offer tailored panels for six different cancer types:

Lung cancer

ALK, ROS1, KRAS, TP53, KEAP1, EGFR, MET, ERBB2, SETD2, RB1, NF1, BRAF, MYC, MGA, CDKN2A

Breast cancer

ESR1, ERBB2, EGFR, CYP19A1, NR3C3, PIK3CA, TP53, MYC, MDM4, AKT1, PTEN, BRCA1, BRCA2, ATM, PALB2, CCND1, KRAS

Bladder cancer

TP53, ARID1A, GATA3, KMT2C, KMT2D, PIK3CA, TSC1, PTEN, RB1, BRAF, CDKN1A, APC, ERBB2, STAG2, KDM6A, BRCA2, BRCA1, ATM, TXNIP

Colorectal cancer

TP53, APC, KRAS, BRAF, MSH2, MSH6, MLH1, POLE, EGFR, ERBB2, ERBB3, ERBB4, FGFR3, MET, ALK, NTRK1, NTRK3

Prostate cancer

AR, TP53, BRCA2, BRCA1, ATM, PALB2, CDK12, PTEN, AKT1, PIK3CA, PIK3R1, SPOP, CHD1, FOXA1, HSD3B1, MSH2, MSH6, MLH1, MYC, CTNNB1, APC

Ovarian cancer

TP53, NF1, BRCA1, BRCA2, ATM, PALB2, CDK12, KIT, MYC, MECOM, TERT, TERC, PIK3CA, CCNE1, KRAS, RB1

Pan-cancer panel

Our pan-cancer panel encompasses 105 genes that are frequently mutated across human cancers. We report somatic mutations and rearrangements in coding regions, a genome-wide copy number profile, and germline variants. Starting price is 2000€ per sample, but discounts apply for larger orders.

Cell cycle

TP53, CDKN2A, CDKN1A, CDKN1B, CDK4, CDK6, CCND1, CCNE1, MDM2, MDM4, MYC, RB1, E2F3, MAP3K1, TP53BP1, STAG2

DNA repair

BRCA1, BRCA2, PALB2, MSH2, MSH6, MLH1, PMS2, ATM, ATR, CHEK2, CDK12, ERCC1, ERCC2, ERCC3, ERCC4, ERCC5, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, RAD51B, RAD51C

PI3K pathway

PTEN, PIK3R1, PIK3CA, PIK3CB, AKT1, AKT2, AKT3, TSC1

WNT pathway

APC, CTNNB1, RNF43, RSPO1

Notch signaling pathway

NOTCH1, NOTCH2, NOTCH3

Growth factor receptors

EGFR, ERBB2, ERBB3, ERBB4, FGFR3, MET

Hormonal signaling

AR, ESR1, CYP19A1, FOXA1, NCOA2

RAS/RAF family

KRAS, NRAS, HRAS, BRAF

Chromatin modifiers

ARID1A, ASXL1, CREBBP, DNMT3A, EP300, IDH1, KDM6A, KMT2C, KMT2D, SETD2, SMARCA1, TET2

Other

BAP1, CHD1, ELF3, FBXW7, FOXP1, GATA3, GNAS, JAK1, KIT, MECOM, MED12, NKX3-1, PPARG, PTCH1, RUNX1, RYBP, SPOP, TBX3, TERT, TXNIP, UTY, XPO1, ZFHX3

Longitudinal analysis

For applications where longitudinal followup of patient tumor burden and clonal dynamics are needed, we offer assays tailored for an individual patient. These assays achieve the highest sensitivity at lowest cost, but are only applicable to patients whose cancer mutation profile is known. After establishing the mutation profile with a cancer specific panel or pan-cancer panel, the price for subsequent longitudinal analysis is 300€ per sample.

Whole exome and whole genome

For samples where our standard panel identifies more than 5% tumor DNA, we provide extended analysis packages which can be purchased at extra cost.

Whole exome analysis

We analyze the blood sample for all somatic mutations and germline variants in coding regions.

Whole genome analysis

We analyze the entire cancer genome, providing a complete view of somatic mutations, copy number changes, and genomic rearrangements.

Contact us

We wish to partner with healthcare providers, academic groups and pharmaceutical companies to provide best possible treatment for patients. Please contact us to discuss how your organization could benefit from partnership with Fluivia.


You can contact us by submitting a contact request below, or by emailing us at contact@fluivia.com.